Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1976 Dec;3(12):3409-21.
doi: 10.1093/nar/3.12.3409.

Nucleotide sequence of the restriction fragments hind L and hind M of SV40 DNA

M Ysebaert, F Thys, A Van de Voorde, W Fiers
Free PMC article

Nucleotide sequence of the restriction fragments hind L and hind M of SV40 DNA

M Ysebaert et al. Nucleic Acids Res. 1976 Dec.
Free PMC article

Abstract

The nucleotide sequence of the two minor SV40 DNA restriction fragments Hind L and Hind M is here reported. For this purpose we used 5'-32p-labeled DNA fragments either partially digested with snake venom diesterase for analysis by the wandering spot method or partially degraded with the base specific reagents dimethylsulphate or hydrazine for direct sequence analysis on gel. In the former procedure the strands were separated before degradation, while in the latter the strands were separated after modification but before the degradation. Due to the presence of several termination codons, the region Hind L - Hind M cannot be translated in a polypeptide. Also, no initiation codons for protein synthesis are present in this region.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Virology. 1975 Nov;68(1):260-5 - PubMed
    1. Eur J Biochem. 1976 Jan 2;61(1):101-17 - PubMed
    1. J Mol Biol. 1973 Aug 5;78(2):363-76 - PubMed
    1. Biochem Biophys Res Commun. 1974 Jul 10;59(1):108-16 - PubMed
    1. Proc Natl Acad Sci U S A. 1974 Dec;71(12):4879-83 - PubMed