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. 1976 Dec;73(12):4551-4.
doi: 10.1073/pnas.73.12.4551.

DNA-relaxing activity and endonuclease activity in Xenopus laevis oocytes

E Mattoccia, D G Attardi, G P Tocchini-Valentini

DNA-relaxing activity and endonuclease activity in Xenopus laevis oocytes

E Mattoccia et al. Proc Natl Acad Sci U S A. 1976 Dec.

Abstract

Complex simian virus 40 DNA produced by a soluble cell-free extract derived from stage 6 oocytes of Xenopus laevis consists of fully relaxed circles (i.e., with no superhelical turns). An endonuclease and a DNA-relaxing protein, either or both of which could be responsible for the relaxation of the complex DNA, have been purified from the extract. The endonuclease(s) produces nicked circles (having a single-strand scission) and linear full-size molecules. The DNA-relaxing protein is in the nucleus, has a molecular weight of apporximately 70,000, and is able to remove both negative and positive superhelical turns.

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References

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