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. 2008 Aug;25(8):375-81.
doi: 10.1007/s10815-008-9250-1. Epub 2008 Sep 23.

Clinical outcome of magnetic activated cell sorting of non-apoptotic spermatozoa before density gradient centrifugation for assisted reproduction

Enver Kerem Dirican  1 , Osman Denizhan Ozgün, Süleyman Akarsu, Kadir Okhan Akin, Ozge Ercan, Mukaddes Uğurlu, Cağri Camsari, Oya Kanyilmaz, Adnan Kaya, Ali Unsal
Affiliations

Clinical outcome of magnetic activated cell sorting of non-apoptotic spermatozoa before density gradient centrifugation for assisted reproduction

Enver Kerem Dirican et al. J Assist Reprod Genet. 2008 Aug.

Abstract

Purpose: Magnetic activated cell sorting (MACS) eliminates apoptotic spermatozoa based on the presence of externalized phosphatidylserine residues. We evaluated the outcome of male fertility treatment when intracytoplasmic sperm injection (ICSI) into human oocytes was performed with non-apoptotic MACS-selected spermatozoa.

Methods: 196 couples were treated by ICSI following spermatozoa preparation by MACS (study group; 122 couples) or density gradient centrifugation (DGC) (control group; 74 couples). Fertilization, cleavage, pregnancy, and implantation rates were analyzed.

Results: The percentage of sperm with normal morphology after MACS selection was improved. Cleavage and pregnancy rates were higher, respectively, in the study group than in control. A slightly higher implantation rate was also observed in the study group.

Conclusions: MACS selection of human spermatozoa increased cleavage and pregnancy rates in oligoasthenozoospermic ART cases. This novel method for selecting non-apoptotic spermatozoa for ICSI is safe and reliable, and may improve the assisted reproduction outcome.

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