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. 2008 Sep-Oct;25(9-10):483-8.
doi: 10.1007/s10815-008-9252-z. Epub 2008 Sep 24.

The effects of different concentrations of sodium selenite on the in vitro maturation of preantral follicles in serum-free and serum supplemented media

A Abedelahi  1 , M Salehnia, A A Allameh
Affiliations

The effects of different concentrations of sodium selenite on the in vitro maturation of preantral follicles in serum-free and serum supplemented media

A Abedelahi et al. J Assist Reprod Genet. 2008 Sep-Oct.

Abstract

Purpose: This study was to investigate the effect of sodium selenite (SS) on in vitro maturation of mouse preantral follicles.

Methods: The isolated preantral follicles were cultured in TCM 199 medium supplemented with different concentrations (0, 5, 10, 15 ng/ml) of SS and 3 mg/ml bovine serum albumin (BSA) or 5% Fetal Bovine Serum (FBS). The ovulation was induced by addition of 1.5 IU/ml human chorionic gonadotropin. The size and development of follicles and oocytes were assessed by calibrated eyepiece.

Results: The survival rates of follicles in FBS supplemented groups containing 5 and 10 ng/ml SS (88.23%, 90.83%) were higher than other groups (P < 0.05 and P < 0.001 respectively). The mean diameter of follicles (199.84 +/- 15.58 microm) and the percentage of MII oocyte (33.08%) were higher in FBS supplemented group containing 10 ng/ml SS (P < 0.001).

Conclusion: The sodium selenite and FBS improve the in vitro growth and maturation of mouse preantral follicles.

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Figures

Fig. 1
Fig. 1
The morphology of mouse preantral follicles during in vitro maturation. The cultured isolated follicle on day 6 (a), day 8 (b) and day 10 of culturing (c). The oocyte ovulated of cultured preantral follicle 16 h after hCG administration (d). As the figure shows the granulosa and thecal cells outgrowth were prominent (Fig. 1a–b) and the antral cavities are seen as clear cavities (Fig. 1c)
Fig. 2
Fig. 2
The follicular diameter (μm) of cultured preantral follicles in serum-free and serum supplemented media containing different concentrations of sodium selenite (ng/ml). a there was significant difference with other groups (P < 0.001). BSA Bovine serum albumin, FBS fetal bovine serum, SS sodium selenite. The day on the figure showed duration after culturing and the first day of culturing was considered as day 0
Fig. 3
Fig. 3
The oocyte diameter (μm) of cultured preantral follicles in serum-free and serum supplemented media containing different concentrations of sodium selenite (ng/ml). a there was significant difference with other groups (P < 0.01). BSA Bovine serum albumin, FBS fetal bovine serum, SS sodium selenite. Note: The day on the figure showed duration after culturing and the first day of culturing was considered as day 0
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