[Abortive myxovirus infection in Ehrlich ascites carcinoma cells. Production of heterokaryons in permissive cells and analysis of virus-specific structures]

Abstract

Hybridization of Ehrlich ascitic carcinoma cells and chicken fibroblasts using UV-inactivated Sendai virus produced the following variants of homo- and heterokaryons: (a) uninfected Ehrlich cells, (b) fowl plague virus-infected chicken fibroblasts, (c) uninfected Ehrlich cells and uninfected chicken fibroblasts; (d) infected Ehrlich cells and infected chicken fibroblasts, (e) infected Ehrlich cells and uninfected chicken fibroblasts. The analysis of the material produced by heterokaryons gave the following results: when Ehrlich ascitic carcinoma cells infected with classical fowl plaque virus and labeled with 3H-uridine fused with uninfected unlabeled chicken fibroblasts, the heterokaryons, in addition to structures with the buoyant density of 1.29 g/ml typical for infected cells, also produced virus structures with a density of 1.25 g/ml and had a higher infectious activity (5.2 X 10(7) PFU/ml) than the structures produced by infected Ehrlich cells (5.8 X 10(6) PFU/ml); after fusion of Ehrlich ascitic carcinoma cells infected with fowl plague virus and labelled with 3H-uridine with unlabelled chicken fibroblasts infected with fowl plague virus heterokaryons produced virus structures with densities of 1.29 g/ml and 1.22 g/ml which had even higher infectious activity (8.6 X 10(8) PFU/ml) than the structures produced by infected Ehlrich ascitic cells.