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Review
. 2008;9(9):234.
doi: 10.1186/gb-2008-9-9-234. Epub 2008 Sep 18.

Transcriptome content and dynamics at single-nucleotide resolution

Nicole Cloonan  1 , Sean M Grimmond
Affiliations
Review

Transcriptome content and dynamics at single-nucleotide resolution

Nicole Cloonan et al. Genome Biol. 2008.

Abstract

Massively parallel short-tag sequencing of cDNA libraries--RNAseq--is being used to study the dynamics and complexity of eukaryotic transcriptomes, giving new biological insights into the 'active genome'.

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Figures

Figure 1
Figure 1
The identification of differential exon splicing by RNAseq. In this hypothetical genome-browser view, RNAseq tags (shown in red) are aligned to the genome sequence, giving a quantitative view of tag densities across the locus. Genome-aligned reads identify individual exons and exon-exon junction usage can be monitored by matching tags to a reference set of junction sequences. Differential exon-exon junction usage can be used to identify canonical and alternative splicing events.
See this image and copyright information in PMC

References

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