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. 2008 Oct 1;13(10):2408-15.
doi: 10.3390/molecules13102408.

Molecular weight and monosaccharide composition of Astragalus polysaccharides

Du-Juan Xu  1 , Quan Xia, Jia-Jia Wang, Pei-Pei Wang
Affiliations

Molecular weight and monosaccharide composition of Astragalus polysaccharides

Du-Juan Xu et al. Molecules. .

Abstract

Two polysaccharides (APS-I and APS-II) were isolated from the water extract of Radix Astragali and purified through ethanol precipitation, deproteination and by ion-exchange and gel-filtration chromatography. Their molecular weight was determined using high performance liquid chromatography and gel permeation chromatography (HPLC-GPC) and their monosaccharide composition was analyzed by TLC and HPLC methods, using a refractive index detector (RID) and an NH(2) column. It was shown that APS-I consisted of arabinose and glucose and APS-II consisted of rhamnose, arabinose and glucose, in a molar ratio of 1:3.45 and 1:6.25:17.86, respectively. The molecular weights (Mw) of APS-I and APS-II were 1,699,100 Da and 1,197,600 Da, respectively.

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Figures

Figure 1
Figure 1
Elution Curve of Astraglus Polysaccharide on DEAE-52 Cellulose.
Figure 2
Figure 2
Elution Curve of APS on Sephadex G-150 column.
Figure 3
Figure 3
HPLC-GPC chromatography of APS-1 and APS-II.
Figure 4
Figure 4
Monosaccharide composition analysis of APS-I and APS-II by TLC.
Figure 5
Figure 5
HPLC-RID analysis of monosaccharide composition.
See this image and copyright information in PMC

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