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Comment
. 2008 Oct 7;105(40):E62; author reply E63-4.
doi: 10.1073/pnas.0804594105. Epub 2008 Oct 1.

Poxvirus transcriptome analysis

P S Satheshkumar, Bernard Moss
Comment

Poxvirus transcriptome analysis

P S Satheshkumar et al. Proc Natl Acad Sci U S A. .
No abstract available

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig. 1.
Fig. 1.
Northern blot showing detection of the same size transcript with D9 and D10 probes. D9 and D10 were previously characterized as early and late genes, respectively (5), but both were designated as immediate–early by Assarsson et al. (1). RNA made at 2 h after mock infection (M) or VACV infection in the presence (+) or absence (−) of cytosine arabinoside (AraC) was detected with 32P-labeled D9 (A) and D10 (B) polyribonucleotide probes. The position of a 1,000-nucleotide RNA marker is shown on the side. The same size transcript was detected with the two probes because the transcription termination signal (T5NT) of ORF D9 occurs within the downstream D10 ORF.
See this image and copyright information in PMC

Comment on

References

    1. Assarsson E, et al. Kinetic analysis of a complete poxvirus transcriptome reveals an immediate–early class of genes. Proc Natl Acad Sci USA. 2008;105:2140–2145. - PMC - PubMed
    1. Niles EG, et al. Nucleotide sequence and genetic map of the 16-kb vaccinia virus Hind III D fragment. Virology. 1986;153:96–112. - PubMed
    1. Boone RF, Parr RP, Moss B. Intermolecular duplexes formed from polyadenylated vaccinia virus RNA. J Virol. 1979;3:365–374. - PMC - PubMed
    1. Ahn B-Y, Rosel J, Cole NB, Moss B. Identification and expression of rpo19, a vaccinia virus gene encoding a 19-kilodalton DNA-dependent RNA polymerase subunit. J Virol. 1992;66:971–982. - PMC - PubMed
    1. Lee-Chen GJ, Niles EG. Structure of the transcription initiation and termination sequences of seven early genes in the vaccinia virus HindIII D fragment. Virology. 1988;163:80–92. - PubMed

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