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. 2009 Jan;32(1):117-9.
doi: 10.2337/dc08-0763. Epub 2008 Oct 3.

Increased matrix metalloproteinase-9 predicts poor wound healing in diabetic foot ulcers

Yu Liu  1 , Danqing Min, Thyra Bolton, Vanessa Nubé, Stephen M Twigg, Dennis K Yue, Susan V McLennan
Affiliations

Increased matrix metalloproteinase-9 predicts poor wound healing in diabetic foot ulcers

Yu Liu et al. Diabetes Care. 2009 Jan.

Abstract

Objective: We studied the relationships of diabetic ulcer wound fluid matrix metalloproteinases (MMPs), tissue inhibitors of metalloproteinases (TIMPs), and transforming growth factor-beta1 (TGF-beta1) with wound healing rate.

Research design and methods: The ulcers were cleansed to remove exudates, and wound fluids were collected for analysis of MMP-2 and -9, TIMP-1, and TGF-beta1.

Results: At presentation, MMP-9 and the MMP-9-to-TIMP-1 ratio correlated inversely with the wound healing rate at 28 days (P < 0.001). MMP-9 and the MMP-9-to-TIMP-1 ratio were lower in the 23 patients who achieved complete healing at 12 weeks versus the 39 who did not. The pro-MMP-9 concentration was predictive of healing within 12 weeks. Addition of cutoffs for TIMP-1 (>480 pg/ml) and TGF-beta (>115 pg/ml) further improved its predictive power (area under the curve 0.94).

Conclusions: These findings suggest that a milieu with high MMP-9 may be indicative of inflammation and poor wound healing. Measurements of MMP-9, TIMP-1, and TGF-beta in wound fluid may help to identify ulcers at risk of poor healing.

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References

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