Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2009 Apr;135(4):559-66.
doi: 10.1007/s00432-008-0490-3. Epub 2008 Oct 8.

HPV-16 E6 L83V variant in squamous cell carcinomas of the upper aerodigestive tract

Paolo Boscolo-Rizzo  1 , Maria Cristina Da Mosto, Roberto Fuson, Helena Frayle-Salamanca, Rossana Trevisan, Annarosa Del Mistro
Affiliations

HPV-16 E6 L83V variant in squamous cell carcinomas of the upper aerodigestive tract

Paolo Boscolo-Rizzo et al. J Cancer Res Clin Oncol. 2009 Apr.

Abstract

Purpose: The aim of this prospective case series study was to determine the prevalence of HPV-DNA, analyze the E6 mRNA expression, identify intra-type variation in the E6 oncogene in upper aerodigestive tract (UADT) squamous cell carcinoma (SCC), and correlate the presence of HPV-DNA with several clinical parameters and outcome.

Methods: Frozen samples of UADT-SCC were analyzed for the presence and characterization of HPV-DNA and RNA sequences by means of polymerase chain reaction (PCR), reverse transcriptase-PCR, and direct sequencing of amplified products.

Results: HPV-DNA sequences were detected in 10% of the tumors, all of which were typed as HPV-16. Positivity for HPV-16 E6/E7 mRNA was observed in five of the eight HPV-positive tumors (62.5%). The HPV-16 E6 L83V variant was present in five cases. Multivariate analysis identified a history of absence of smoking (P = 0.009) as a predictor of HPV-positive tumor. No significant differences in overall and disease free survival curves were observed between patients with HPV-positive tumors and patients with tumors without detectable HPV-DNA.

Conclusion: Our findings support the etiological participation of HPV-16 in a subset of UADT-SCCs from patients lacking traditional risk factors. The potential prognostic significance of HPV-16 E6 L83V variant in HPV-16 positive UADT-SCCs should be more extensively investigated.

PubMed Disclaimer

References

    1. Badaracco G, Rizzo C, Mafera B, Pichi B, Giannarelli D, Rahimi SS et al (2007) Molecular analyses and prognostic relevance of HPV in head and neck tumours. Oncol Rep 17:931–939 - PubMed
    1. Barra S, Franceschi S, Negri E, Talamini R, La Vecchia C (1990) Type of alcoholic beverage and cancer of the oral cavity, pharynx and oesophagus in an Italian area with high wine consumption. Int J Cancer 46:1017–1020. doi:10.1002/ijc.2910460612 - DOI - PubMed
    1. Bosch FX, de Sanjosé S (2002) Human papillomavirus in cervical cancer. Curr Oncol Rep 4:175–183. doi:10.1007/s11912-002-0079-y - DOI - PubMed
    1. Braakhuis BJ, Snijders PJ, Keune WJ, Meijer CJ, Ruijter-Schippers HJ, Leemans CR et al (2004) Genetic patterns in head and neck cancers that contain or lack transcriptionally active human papillomavirus. J Natl Cancer Inst 96:998–1006 - DOI - PubMed
    1. Butz K, Geisen C, Ullmann A, Spitkovsky D, Hoppe-Seyler F (1996) Cellular responses of HPV-positive cancer cells to genotoxic anti-cancer agents: repression of E6/E7-oncogene expression and induction of apoptosis. Int J Cancer 68:506–513. doi:10.1002/(SICI)1097-0215(19961115)68:4<506::AID-IJC17>3.0.CO;2-2 - DOI - PubMed

MeSH terms