Metabolic regulation and relationship of endogenous protein kinase activity and steroidogenesis in isolated adrenocortical carcinoma cells of the rat
- PMID: 188548
Metabolic regulation and relationship of endogenous protein kinase activity and steroidogenesis in isolated adrenocortical carcinoma cells of the rat
Abstract
In the adrenocortical carcinoma cell, in contrast to normal isolated adrenal cells, 10 to 50 muunits of ACTH do not raise the level of adenosine cyclic 3':5'-monophosphate (cyclic AMP), protein kinase activity, and steroidogenesis. This indicates a lesion in the tumor adenylate cyclase system. Two-tenths to 10 mM cyclic AMP and guanosine cyclic 3':5'-monophosphate (cyclic GMP) which stimulate steroidogenesis in a normal cell, activate protein kinase activity in a concentration-response manner without any detectable rise in steroidogenesis in the adrenocortical carcinoma cell. Cycloheximide and actinomycin D do not inhibit the stimulation of the phosphorylation. These results suggest that the tumor cyclic nucleotide-dependent protein kinase activity is unrelated to steroidogenesis and is also not under the transcriptional or translational control steps. Curiously, muM concentrations of cyclic AMP, in contrast to cyclic GMP, stimulate protein kinase activity. In a normal cell, both cyclic AMP and cyclic GMP, in this concentration range, stimulate protein kinase without an increase in steroidogenesis. It is therefore proposed that, in contrast to the normal cell, there is an additional defect in cyclic GMP-dependent protein kinase.
Similar articles
-
Role of cyclic AMP and protein kinase on the steroidogenic action of ACTH, prostaglandin E1 and dibutyryl cyclic AMP in normal adrenal cells and adrenal tumor cells from humans.J Cyclic Nucleotide Res. 1978 Aug;4(4):311-21. J Cyclic Nucleotide Res. 1978. PMID: 214468
-
Metabolic regulation of steroidogenesis in isolated adrenal cells of rat. Relationship of adrenocorticotropin-, adenosine 3':5'-monophosphate-and guanosine 3':5'-monophosphate-stimulated steroidogenesis with the activation of protein kinase.Eur J Biochem. 1976 Nov 15;70(2):427-33. doi: 10.1111/j.1432-1033.1976.tb11033.x. Eur J Biochem. 1976. PMID: 188647
-
Partial purification and characterization of the defective cyclic adenosine 3':5'-monophosphate binding protein kinase from adrenocortical carcinoma.Cancer Res. 1977 Sep;37(9):3297-300. Cancer Res. 1977. PMID: 195724
-
[Cyclic nucleotides as intracellular transmitters of hormone action].Usp Sovrem Biol. 1975 Nov-Dec;80(3):351-69. Usp Sovrem Biol. 1975. PMID: 3057 Review. Russian. No abstract available.
-
[Adrenocorticotrophic hormone (ACTH)].Horumon To Rinsho. 1971 Oct;19(10):796-803. Horumon To Rinsho. 1971. PMID: 4113149 Review. Japanese. No abstract available.
Cited by
-
ACTH-Modulated Membrane Guanylate Cyclase Signaling System: Origin and Creation.Front Mol Neurosci. 2022 Aug 9;15:929396. doi: 10.3389/fnmol.2022.929396. eCollection 2022. Front Mol Neurosci. 2022. PMID: 36017080 Free PMC article. Review.
-
Membrane guanylate cyclase, a multimodal transduction machine: history, present, and future directions.Front Mol Neurosci. 2014 Jul 2;7:56. doi: 10.3389/fnmol.2014.00056. eCollection 2014. Front Mol Neurosci. 2014. PMID: 25071437 Free PMC article. Review.