Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1991 Sep;173(17):5589-91.
doi: 10.1128/jb.173.17.5589-5591.1991.

Escherichia coli orfE (upstream of pyrE) encodes RNase PH

K A Ost  1 , M P Deutscher
Affiliations

Escherichia coli orfE (upstream of pyrE) encodes RNase PH

K A Ost et al. J Bacteriol. 1991 Sep.

Abstract

RNase PH from extracts of Escherichia coli was purified to homogeneity and subjected to NH2-terminal sequencing. Comparison of this sequence with all open reading frames in the GenBank data base revealed at least 95% identity to an unidentified open reading frame (orfE) upstream of pyrE at 81.7 min on the E. coli chromosome. Clones of orfE overexpress RNase PH activity, verifying that orfE encodes this ribonuclease. We suggest that orfE be renamed rph.

PubMed Disclaimer

References

    1. Mol Microbiol. 1989 Mar;3(3):393-404 - PubMed
    1. J Biol Chem. 1988 Jan 25;263(3):1518-23 - PubMed
    1. Gene. 1985;33(1):103-19 - PubMed
    1. Nucleic Acids Res. 1985 Jun 11;13(11):4113-23 - PubMed
    1. EMBO J. 1984 Aug;3(8):1783-90 - PubMed

Publication types