In vitro activity of the nitrogen fixation regulatory protein FIXJ from Rhizobium meliloti
- PMID: 1885556
- PMCID: PMC208327
- DOI: 10.1128/jb.173.18.5914-5917.1991
In vitro activity of the nitrogen fixation regulatory protein FIXJ from Rhizobium meliloti
Abstract
Cell extracts of an Escherichia coli strain that overproduces the regulatory protein FIXJ from Rhizobium meliloti promoted transcription of fixK, a known FIXJ-dependent gene, in a coupled transcription-translation assay. Activation by FIXJ was dependent on the sigma 70 holoenzyme form of RNA polymerase.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
