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. 1991 Sep;173(18):5914-7.
doi: 10.1128/jb.173.18.5914-5917.1991.

In vitro activity of the nitrogen fixation regulatory protein FIXJ from Rhizobium meliloti

J Batut  1 , E Santero, S Kustu
Affiliations

In vitro activity of the nitrogen fixation regulatory protein FIXJ from Rhizobium meliloti

J Batut et al. J Bacteriol. 1991 Sep.

Abstract

Cell extracts of an Escherichia coli strain that overproduces the regulatory protein FIXJ from Rhizobium meliloti promoted transcription of fixK, a known FIXJ-dependent gene, in a coupled transcription-translation assay. Activation by FIXJ was dependent on the sigma 70 holoenzyme form of RNA polymerase.

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