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. 1991 Sep 5;266(25):16448-52.

Functional cloning of a Dictyostelium discoideum cDNA encoding GMP synthetase

M M Van Lookeren Campagne  1 , J Franke, R H Kessin
Affiliations
  • PMID: 1885577
Free article

Functional cloning of a Dictyostelium discoideum cDNA encoding GMP synthetase

M M Van Lookeren Campagne et al. J Biol Chem. .
Free article

Abstract

A functional cloning procedure has been used to recover a cDNA coding for the GMP synthetase of Dictyostelium discoideum. The enzyme is encoded by a single gene, which is actively transcribed during growth, but not during development. The open reading frame encodes a protein of 718 amino acids with a predicted molecular mass of 79.6 kDa. The Dictyostelium enzyme has extensive homology with the GMP synthetase of Escherichia coli and regional homology to other glutamine amidotransferases.

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