The use of native thick filaments in in vitro motility assays
- PMID: 1885662
- DOI: 10.1242/jcs.1991.supplement_14.14
The use of native thick filaments in in vitro motility assays
Abstract
Native thick filaments from the clam, Mercinaria mercinaria translocate actin filaments both toward and away from the center of the thick filament in an in vitro motility assay. The thick filaments from the adductor muscle are about 10 microns long whereas those from the catch muscle are 30-50 microns long. These thick filaments should prove useful in understanding the mechanism of myosin-dependent movement of actin filaments.
Similar articles
-
Modification of the bi-directional sliding movement of actin filaments along native thick filaments isolated from a clam.J Muscle Res Cell Motil. 1996 Dec;17(6):637-46. doi: 10.1007/BF00154058. J Muscle Res Cell Motil. 1996. PMID: 8994083
-
Mg-ATPase activity and motility of native thick filaments isolated from the anterior byssus retractor muscle of Mytilus edulis.J Muscle Res Cell Motil. 1989 Apr;10(2):124-34. doi: 10.1007/BF01739968. J Muscle Res Cell Motil. 1989. PMID: 2527245
-
Bi-directional movement of actin filaments along long bipolar tracks of oriented rabbit skeletal muscle myosin molecules.FEBS Lett. 1997 Jun 16;409(3):380-4. doi: 10.1016/s0014-5793(97)00558-9. FEBS Lett. 1997. PMID: 9224694
-
Direction and speed of actin filaments moving along thick filaments isolated from molluscan smooth muscle.J Biochem. 1990 Sep;108(3):341-3. doi: 10.1093/oxfordjournals.jbchem.a123203. J Biochem. 1990. PMID: 2277026 Review.
-
Comparative biomechanics of thick filaments and thin filaments with functional consequences for muscle contraction.J Biomed Biotechnol. 2010;2010:473423. doi: 10.1155/2010/473423. Epub 2010 Jun 6. J Biomed Biotechnol. 2010. PMID: 20625489 Free PMC article. Review.
Cited by
-
Loss of actomyosin regulation in distal arthrogryposis myopathy due to mutant myosin binding protein-C slow.FASEB J. 2013 Aug;27(8):3217-28. doi: 10.1096/fj.13-228882. Epub 2013 May 8. FASEB J. 2013. PMID: 23657818 Free PMC article.
-
Stepwise sliding of single actin and Myosin filaments.Biophys J. 2004 Jan;86(1 Pt 1):353-8. doi: 10.1016/S0006-3495(04)74111-9. Biophys J. 2004. PMID: 14695277 Free PMC article.
-
Coordinated force generation of skeletal myosins in myofilaments through motor coupling.Nat Commun. 2017 Jul 6;8:16036. doi: 10.1038/ncomms16036. Nat Commun. 2017. PMID: 28681850 Free PMC article.
-
Stepwise length changes in single invertebrate thick filaments.Biophys J. 2005 Nov;89(5):3269-76. doi: 10.1529/biophysj.105.069864. Epub 2005 Aug 19. Biophys J. 2005. PMID: 16113114 Free PMC article.