Miniaturization of three carbohydrate analyses using a microsample plate reader
- PMID: 1888021
- DOI: 10.1016/0003-2697(91)90300-i
Miniaturization of three carbohydrate analyses using a microsample plate reader
Abstract
Three carbohydrate analyses (reducing value by copper-bicinchoninate, total carbohydrate by phenol-sulfuric acid, and D-glucose by glucose oxidase) have been miniaturized using a microsample plate reader. The use of the reducing-value procedure to measure the hydrolysis of starch by alpha-amylase and the use of the glucose oxidase method to measure the hydrolysis of lactose by lactase are illustrated.
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