Kinetic studies on immune hemolysis; a method
- PMID: 18884902
- PMCID: PMC2135836
- DOI: 10.1084/jem.88.4.427
Kinetic studies on immune hemolysis; a method
Abstract
A quantitative method for the study of the kinetics of immune hemolysis is described. In the presence of excess C' and limited amounts of antibody the kinetics of hemolysis resemble those of enzymatic processes. Assays of hemolytic antibody should therefore be based on velocity measurements rather than end point titrations.
Similar articles
-
Kinetic studies on immune hemolysis. II. The reversibility of red cell-antibody combination and the resultant transfer of antibody from cell to cell during hemolysis.J Exp Med. 1951 Aug;94(2):87-110. doi: 10.1084/jem.94.2.87. J Exp Med. 1951. PMID: 14861372 Free PMC article.
-
Chronic hemolytic anemia due to cold agglutinins: the mechanism of resistance of red cells to C' hemolysis by cold agglutinins.J Clin Invest. 1967 Sep;46(9):1461-74. doi: 10.1172/JCI105638. J Clin Invest. 1967. PMID: 4166664 Free PMC article.
-
Studies on immune human hemolysis. I. The kinetics of the Donath-Land-steiner reaction and the requirement for complement in the reaction.J Exp Med. 1961 Jan 1;113(1):177-218. doi: 10.1084/jem.113.1.177. J Exp Med. 1961. PMID: 13714487 Free PMC article.
-
Oxidative stress in paroxysmal nocturnal hemoglobinuria and other conditions of complement-mediated hemolysis.Free Radic Biol Med. 2015 Nov;88(Pt A):63-9. doi: 10.1016/j.freeradbiomed.2015.04.027. Epub 2015 Apr 30. Free Radic Biol Med. 2015. PMID: 25937178 Review.
-
Complement "specificity" and interchangeability: measurement of hemolytic complement levels and use of the complement-fixation test with sera from common domesticated animals.Am J Vet Res. 1977 Oct;38(10):1611-7. Am J Vet Res. 1977. PMID: 337860 Review.
Cited by
-
[The influence of homologous serum of tuberculin cytolysis after treatment of the serum and cell donor].Z Med Mikrobiol Immunol. 1966 Apr 25;152(2):140-7. Z Med Mikrobiol Immunol. 1966. PMID: 5990051 German. No abstract available.
-
[The microflocculation reaction, on slides using the agar double diffusion technic, between poliomyelitis antigens and antibodies. II. Comparative study of the neutralizing, complement-fixing and flocculation antibodies].Arch Gesamte Virusforsch. 1960;10:351-60. Arch Gesamte Virusforsch. 1960. PMID: 13731895 French. No abstract available.
-
[Methodological and clinical studies on the properdin system. III. Studies on the behavior of 4 complement components after major gynecological operations].Arch Gynakol. 1961;196:405-22. doi: 10.1007/BF00669727. Arch Gynakol. 1961. PMID: 13909570 German. No abstract available.
-
[On the microscopic demonstration of the complement-fixating antigen-antibody complex with fluorescein-labelled anti-complement].Z Zellforch Microsk Anat Histochem. 1961;2:176-85. doi: 10.1007/BF00737545. Z Zellforch Microsk Anat Histochem. 1961. PMID: 13696230 German. No abstract available.
-
A STUDY OF THE CROSS-REACTIVITY OF ANTIPURIN-6-OYL SERUM WITH DEOXYRIBONUCLEIC ACID (DNA).J Exp Med. 1965 Jan 1;121(1):19-38. doi: 10.1084/jem.121.1.19. J Exp Med. 1965. PMID: 14253484 Free PMC article.