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. 1991 Jul 22;103(2):243-7.
doi: 10.1016/0378-1119(91)90280-o.

Cloning and sequencing of the gilthead seabream (Sparus aurata) growth hormone-encoding cDNA

B Funkenstein  1 , T T Chen, D A Powers, B Cavari
Affiliations

Cloning and sequencing of the gilthead seabream (Sparus aurata) growth hormone-encoding cDNA

B Funkenstein et al. Gene. .

Abstract

The cDNA clones encoding gilthead seabream (gsb) (Sparus aurata) growth hormone (GH) have been isolated from a cDNA library prepared from seabream pituitary gland poly(A)+ RNA. The cDNA library was screened using red seabream and rainbow trout GH cDNAs. The complete nucleotide (nt) sequence of gsbGH has been determined. The cDNA sequence codes for a polypeptide of 204 amino acids (aa), including a putative signal peptide of 17 aa. The 5'- and 3'-untranslated regions of the message are 55 and 236 nt long, respectively. The predicted aa sequence of gsbGH revealed 97% homology with red seabream GH, 95% with tuna GH, 85% with yellowtail GH, and 65% with rainbow trout GH.

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