Dystrophin in control and mdx retina

Abstract

To determine whether or not dystrophin really exists in the outer plexiform layer (OPL) of the retina, we studied control and mdx mice, using four kinds of polyclonal antibodies (DMDP-II, 60 kd, 30 kd and DMDP-IV) against dystrophin. Although control OPL showed a positive immunohistochemical reaction with all four antibodies, mdx OPL showed a positive reaction with DMDP-II and DMDP-IV, a negative reaction with 60 kd and 30 kd antibodies. Immunoblot analysis showed the positive band compatible with the immunohistochemical reaction.