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. 1991 Jul;57(7):2097-100.
doi: 10.1128/aem.57.7.2097-2100.1991.

Rapid and sensitive assay for the phytotoxin rhizobitoxine

X Ruan  1 , N K Peters
Affiliations

Rapid and sensitive assay for the phytotoxin rhizobitoxine

X Ruan et al. Appl Environ Microbiol. 1991 Jul.

Abstract

Rhizobitoxine is a phytotoxin synthesized by some strains of the legume symbiont genus Bradyrhizobium and the plant pathogen Pseudomonas andropogonis. We demonstrate here a new enzymatic assay which is 100-fold more sensitive than previous assays and can detect as little as 1.0 pmol of rhizobitoxine. The assay is based on the inhibition of Salmonella typhimurium beta-cystathionase by rhizobitoxine. Interestingly, beta-cystathionase from Bradyrhizobium japonicum is insensitive to rhizobitoxine at concentrations lower than 75 microM.

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