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. 2008;47(46):8889-92.
doi: 10.1002/anie.200803508.

Probing the aglycon promiscuity of an engineered glycosyltransferase

Richard W Gantt  1 , Randal D Goff, Gavin J Williams, Jon S Thorson
Affiliations

Probing the aglycon promiscuity of an engineered glycosyltransferase

Richard W Gantt et al. Angew Chem Int Ed Engl. 2008.
No abstract available

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Figures

Figure 1
Figure 1
a) Percent conversion of each library member with both WT and ‘ASP’ OleD. Members are listed in descending order of ‘ASP’ conversion with numbering corresponding to the structures listed in (b). b) Structures of the corresponding library members. Compounds leading to trace products (58–73, >5% conversion) or no conversion (74–139) are listed in the Supplementary Information.
Figure 2
Figure 2
Products isolated from large scale enzymatic reactions of ‘ASP’ OleD with (a) phenol (8), (b) thiophenol (6), and (c) aniline (34).
Scheme 1
Scheme 1
a) Natural reaction catalyzed by WT OleD b) General reaction for probing aglycon promiscuity in vitro against a panel of 137 library members, X = OH, SH, NH2, or NHR.
See this image and copyright information in PMC

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