Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2009 Feb 13;284(7):4047-51.
doi: 10.1074/jbc.R800054200. Epub 2008 Oct 16.

Ryanodine receptor structure: progress and challenges

Susan L Hamilton  1 , Irina I Serysheva
Affiliations
Review

Ryanodine receptor structure: progress and challenges

Susan L Hamilton et al. J Biol Chem. .
No abstract available

PubMed Disclaimer

Figures

FIGURE 1.
FIGURE 1.
Correlating primary sequence motifs with subdomains in the 9.6-Å three-dimensional structure. A, sites identified in the primary sequence of RyR1. DHPR, dihydropyridine receptor; MH, malignant hyperthermia; CCD, central core disease; mMC, multiminicore disease. B, cryo-EM map viewed from the cytoplasm (upper panel) and from the sarcoplasmic reticulum (SR) lumen (lower panel). RyR1 subunits are color-coded; putative subregions are indicated. Circles indicate the ligand-binding sites and RyR sequences on the three-dimensional map of RyR by single-particle cryo-EM: red, CaM-binding site; purple, FKBP12-binding site; magenta, imperatoxin A (IpTxA)-binding site; green, sequences mapped by reconstructions of expressed RyR2-GFP fusion proteins; cyan, sequence-specific antibody-binding sites; yellow, the position of the glutathione S-transferase tag fused to the N terminus of RyR3. C, enlargement of the boxed region in B. Ligand-binding sites are labeled as described for B.
FIGURE 2.
FIGURE 2.
RyR1 Ca2+ release channel closed-state structure at 9.6-Å resolution (2, 4). A, two subunits of RyR1 are shown as semitransparent surfaces. α-Helices in the Ca2+ conduction pathway of the TM region are annotated. SR, sarcoplasmic reticulum. B, malignant hyperthermia/central core disease mutations are mapped to the homology models for the N-terminal region of RyR1 (aa 12–565), localized to the clamp-shaped region of the channel structure (aa numbers correspond to Swiss-Prot accession number P11716). The FKBP12-binding region is indicated in green; the LZ1 region is shown in blue; and hyper-reactive cysteines (Cys36 and Cys315) are indicated in magenta. The location of Cys315 (magenta circle) is tentatively assigned because sequence Ala311–Glu343 was excluded from the homology model due to lack of a structural template for this region.
See this image and copyright information in PMC

References

    1. Serysheva II, Chiu W, Ludtke SJ. Methods Cell Biol. 2007;79:407–435. - PubMed
    1. Ludtke SJ, Serysheva II, Hamilton SL, Chiu W. Structure (Camb) 2005;13:1203–1211. - PMC - PubMed
    1. Samso M, Wagenknecht T, Allen PD. Nat Struct Mol Biol. 2005;12:539–544. - PMC - PubMed
    1. Serysheva II, Ludtke SJ, Baker ML, Cong Y, Topf M, Eramian D, Sali A, Hamilton SL, Chiu W. Proc Natl Acad Sci U S A. 2008;105:9610–9615. - PMC - PubMed
    1. Block BA, Franzini-Armstrong C. J Cell Biol. 1988;107:1099–1112. - PMC - PubMed

MeSH terms

Substances

LinkOut - more resources