Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2008 Oct 1;64(Pt 10):954-6.
doi: 10.1107/S1744309108028522. Epub 2008 Sep 30.

Initial crystallographic study of human PCNA in complex with a peptide containing the noncanonical PIP-box sequence of human DNA polymerase iota

Asami Hishiki  1 , Toshiyuki Shimizu, Tomo Hanafusa, Haruo Ohmori, Mamoru Sato, Hiroshi Hashimoto
Affiliations

Initial crystallographic study of human PCNA in complex with a peptide containing the noncanonical PIP-box sequence of human DNA polymerase iota

Asami Hishiki et al. Acta Crystallogr Sect F Struct Biol Cryst Commun. .

Abstract

Human DNA polymerase iota (Poliota) is one of the Y-family DNA polymerases involved in translesion synthesis (TLS), which allows continued replication at damaged DNA templates. Poliota has a noncanonical PCNA-interacting protein box (PIP-box) within an internal region of the protein. Poliota activity is stimulated by PCNA binding through the noncanonical PIP-box. To clarify the interaction of PCNA with the noncanonical PIP-box of Poliota, PCNA and a Poliota peptide carrying the noncanonical PIP-box complex have been cocrystallized. The crystal belongs to space group C2, with unit-cell parameters a = 167.1, b = 68.7, c = 90.0 A, beta = 95.1 degrees . Structural analysis by molecular replacement is in progress.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Crystals of the PCNA–Polι peptide complex.
Figure 2
Figure 2
A diffraction pattern from a crystal of the PCNA–Polι peptide complex.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Bruning, J. B. & Shamoo, Y. (2004). Structure, 12, 2209–2219. - PubMed
    1. Fukuda, K., Morioka, H., Imajou, S., Ikeda, S., Ohtsuka, E. & Tsurimoto, T. (1995). J. Biol. Chem.270, 22527–22534. - PubMed
    1. Gulbis, J. M., Kelman, Z., Hurwitz, J., O’Donnell, M. & Kuriyan, J. (1996). Cell, 87, 297–306. - PubMed
    1. Haracska, L., Johnson, R. E., Unk, I., Phillips, B., Hurwitz, J., Prakash, L. & Prakash, S. (2001a). Mol. Cell. Biol.21, 7199–7206. - PMC - PubMed
    1. Haracska, L., Johnson, R. E., Unk, I., Phillips, B., Hurwitz, J., Prakash, L. & Prakash, S. (2001b). Proc. Natl Acad. Sci. USA, 98, 14256–14261. - PMC - PubMed

Publication types

MeSH terms