Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2009:464:267-83.
doi: 10.1007/978-1-60327-461-6_15.

Detection of the nuclear poly(ADP-ribose)-metabolizing enzymes and activities in response to DNA damage

Jean-Christophe Amé  1 , Antoinette Hakmé, Delphine Quenet, Elise Fouquerel, Françoise Dantzer, Valérie Schreiber
Affiliations

Detection of the nuclear poly(ADP-ribose)-metabolizing enzymes and activities in response to DNA damage

Jean-Christophe Amé et al. Methods Mol Biol. 2009.

Abstract

Poly(ADP-ribosyl)ation is a posttranslational modification of proteins in higher eukaryotes mediated by poly(ADP-ribose) polymerases (PARPs) that is involved in many physiological processes such as DNA repair, transcription, cell division, and cell death. Biochemical studies together with PARP-1- or PARP-2-deficient cellular and animal models have revealed the redundant but also complementary functions of the two enzymes in the surveillance and maintenance of genome integrity. Poly(ADP-ribose) is degraded by the endo- and exo-glycosidase activities of poly(ADP-ribose) glycohydrolase (PARG). In this chapter, biochemical and immunofluorescence methods are described for detecting and assaying PARPs and PARG.

PubMed Disclaimer

MeSH terms

Substances

LinkOut - more resources