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. 1991 Sep 15;88(18):7978-82.
doi: 10.1073/pnas.88.18.7978.

Assembly of combinatorial antibody libraries on phage surfaces: the gene III site

C F Barbas 3rd  1 , A S Kang, R A Lerner, S J Benkovic
Affiliations

Assembly of combinatorial antibody libraries on phage surfaces: the gene III site

C F Barbas 3rd et al. Proc Natl Acad Sci U S A. .

Abstract

A phagemid system was developed for the monovalent display of combinatorial antibody Fab libraries on the surface of filamentous phage M13. Fab fragments were fused to the carboxyl-terminal domain of the gene III protein. Phage displaying Fab fragments on their surface, or Phabs, were enriched by 10(3)- to 10(5)-fold on antigen-coated surfaces over nonspecific phage. The method may replace current antibody cloning techniques.

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