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. 2008 Nov;14(11):1784-6.
doi: 10.3201/eid1411.080589.

Metagenomic diagnosis of bacterial infections

Shota Nakamura  1 , Norihiro Maeda, Ionut Mihai Miron, Myonsun Yoh, Kaori Izutsu, Chidoh Kataoka, Takeshi Honda, Teruo Yasunaga, Takaaki Nakaya, Jun Kawai, Yoshihide Hayashizaki, Toshihiro Horii, Tetsuya Iida
Affiliations

Metagenomic diagnosis of bacterial infections

Shota Nakamura et al. Emerg Infect Dis. 2008 Nov.

Abstract

To test the ability of high-throughput DNA sequencing to detect bacterial pathogens, we used it on DNA from a patient's feces during and after diarrheal illness. Sequences showing best matches for Campylobacter jejuni were detected only in the illness sample. Various bacteria may be detectable with this metagenomic approach.

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Figure
Figure
Comparison of the organisms from which the best matches for the sequences were derived from a BLASTN (http://blast.ncbi.nlm.nih.gov) search with an expect-value cutoff of 10–5. A) DNA from nondiarrheic fecal sample collected 3 months after patient had recovered. B) DNA from diarrheic fecal sample collected while patient was ill.
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