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. 2009 Jan;42(1-2):57-63.
doi: 10.1016/j.clinbiochem.2008.09.116. Epub 2008 Oct 17.

Glutathione S-transferases related to P. aeruginosa lung infection in cystic fibrosis children: preliminary study

Marie-Noëlle Feuillet-Fieux  1 , Thao Nguyen-Khoa, Marie-Anne Loriot, Mairead Kelly, Philippe de Villartay, Isabelle Sermet, Patricia Verrier, Jean-Paul Bonnefont, Philippe Beaune, Gérard Lenoir, Bernard Lacour
Affiliations

Glutathione S-transferases related to P. aeruginosa lung infection in cystic fibrosis children: preliminary study

Marie-Noëlle Feuillet-Fieux et al. Clin Biochem. 2009 Jan.

Abstract

Objectives: In cystic fibrosis (CF) children, we investigated the predictive impact of glutathione S-transferases (GST) activity and genotypes P1, M1 and T1, and antioxidant levels on stage-severity of Pseudomonas aeruginosa lung infection.

Methods: GST activity was determined in whole blood by spectrophotometry, and GST genotypes by multiplex PCR RFLP for 36 CF and 9 control children. Levels of glutathione in erythrocyte and vitamins A, E and C in plasma were measured by HPLC.

Results: No difference in GST activity and no relationship between GST activity and antioxidant levels were observed in CF children as compared to controls. However, GST activity was lower in CF children with severe clinical status and infection, and the frequency of GSTP1 wild type genotype AA, prevalent in uninfected CF children (75%), decreased in infected ones (33%).

Conclusion: GST activity and genotype could play an important role in modulating P. aeruginosa lung infection in CF patients.

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