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Case Reports
. 2008 Nov 20;182(1-3):e1-6.
doi: 10.1016/j.forsciint.2008.08.012. Epub 2008 Oct 30.

Case report: Etizolam and its major metabolites in two unnatural death cases

Takuma Nakamae  1 , Tatsuo Shinozuka, Chizuko Sasaki, Akira Ogamo, Chikako Murakami-Hashimoto, Wataru Irie, Masaru Terada, Shigeki Nakamura, Masataka Furukawa, Katsuyoshi Kurihara
Affiliations
Case Reports

Case report: Etizolam and its major metabolites in two unnatural death cases

Takuma Nakamae et al. Forensic Sci Int. .

Abstract

A simultaneous analytical method for etizolam and its main metabolites (alpha-hydroxyetizolam and 8-hydroxyetizolam) in whole blood was developed using solid-phase extraction, TMS derivatization and ion trap gas chromatography tandem mass spectrometry (GC-MS/MS). Separation of etizolam, TMS derivatives of alpha-hydroxyetizolam and 8-hydroxyetizolam and fludiazepam as internal standard was performed within about 17 min. The inter-day precision evaluated at the concentration of 50 ng/mL etizolam, alpha-hydroxyetizolam and 8-hydroxyetizolam was evaluated 8.6, 6.4 and 8.0% respectively. Linearity occurred over the range in 5-50 ng/mL. This method is satisfactory for clinical and forensic purposes. This method was applied to two unnatural death cases suspected to involve etizolam. Etizolam and its two metabolites were detected in these cases.

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