Diagnosis of human hepatitis B virus in non-treated blood by the bovine IgG DNA-linked carbon nanotube biosensor

Abstract

Objectives: To identify an effective bioassay for human hepatitis B virus (HBV) using voltammetric sensor.

Study design: Electrode was prepared with bovine IgG immobilized onto a DNA-linked carbon nanotube electrode (BIDCE). Analytical BIDCE conditions were optimized through square wave (SW) stripping and cyclic voltammetry (CV).

Results: Optimum parameters of 75 Hz SW frequency, 0.1 V SW amplitude, -1.3 V SW accumulation potential, 100 s accumulation time, and 3.0 mV SW increment potential were obtained. Within working ranges of 0.035-0.242 mg/mL anti-bovine IgG, the relative standard deviation of 0.2 mL HBV was observed to be 0.04 (n=4).

Conclusions: Diagnostic application was performed through direct assay of HBV in non-treated human blood.