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Review
. 2008 Dec;43(4):372-5.
doi: 10.1016/j.jcv.2008.08.020. Epub 2008 Nov 1.

Using multiplex real time PCR in order to streamline a routine diagnostic service

R N Gunson  1 , S Bennett, A Maclean, W F Carman
Affiliations
Review

Using multiplex real time PCR in order to streamline a routine diagnostic service

R N Gunson et al. J Clin Virol. 2008 Dec.

Abstract

An increasing number of virology laboratories are now utilising in house real time PCR assays as the frontline diagnostic tests. As the number of tests on offer increases the natural progression from this will be to rationalise their service via multiplexing. Since 2003 we have introduced a large number of qualitative and quantitative multiplex real time PCR assays into our routine testing service. This paper describes the development of the multiplex assays, the problems encountered and the resultant benefits to the routine service.

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Figures

Fig. 1
Fig. 1
ABI 7500 trace showing appearance of crosstalk in the Cy5 channel (shown by arrow) that appeared when using tamra-bidopy as the fourth dye (A). The un-arrowed trace is the Cy5 positive control. Diagram (B) shows that the crosstalk is gone when ROX is used as the fourth dye (the remaining trace is the Cy5 positive control).
See this image and copyright information in PMC

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