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. 2007:(5):230.
doi: 10.3791/230. Epub 2007 Jul 4.

Protocol for RNAi assays in adult mosquitoes (A. gambiae)

Lindsey Garver  1 , George Dimopoulos
Affiliations

Protocol for RNAi assays in adult mosquitoes (A. gambiae)

Lindsey Garver et al. J Vis Exp. 2007.

Abstract

Reverse genetic approaches have proven extremely useful for determining which genes underly resistance to vector pathogens in mosquitoes. This video protocol illustrates a method used by the Dimopoulos lab to inject dsRNA into Anopheles gambiae mosquitoes, which harbor the malaria parasite. The technique manipulating the injection setup and injecting dsRNA into the thorax is illustrated.

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References

    1. Boisson , et al. 2006
    1. Blandin , et al. 2002

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