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. 2009;43(1-3):198-209.
doi: 10.1007/s12026-008-8071-8.

Redox warfare between airway epithelial cells and Pseudomonas: dual oxidase versus pyocyanin

Balázs Rada  1 , Thomas L Leto
Affiliations

Redox warfare between airway epithelial cells and Pseudomonas: dual oxidase versus pyocyanin

Balázs Rada et al. Immunol Res. 2009.

Abstract

The importance of reactive oxygen species-dependent microbial killing by the phagocytic cell NADPH oxidase has been appreciated for some time, although only recently has an appreciation developed for the partnership of lactoperoxidase with related dual oxidases (Duox) within secretions of the airway surface layer. This system produces mild oxidants designed for extracellular killing that are effective against several airway pathogens, including Staphylococcus aureus, Burkholderia cepacia, and Pseudomonas aeruginosa. Establishment of chronic pseudomonas infections involves adaptations to resist oxidant-dependent killing by expression of a redox-active virulence factor, pyocyanin, that competitively inhibits epithelial Duox activity by consuming intracellular NADPH and producing superoxide, thereby inflicting oxidative stress on the host.

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Figures

Fig.1
Fig.1. The oxidative antimicrobial Duox/SCN-/LPO-system of major airways and counteroffensive mechanisms of Pseudomonas aeruginosa imposed by the redox active virulence factor pyocyanin
A) Lactopreoxidase is produced in the submucosal glands of the airways and accumulates in the airway surface layer, where it converts thiocyanate into microbicidal hypothiocanite using Duox-derived hydrogen peroxide. B) The redox-active Pseudomonas pigment, pyocyanin, enters airway cells, inhibits Duox activity by competing for its substrate (NADPH), and by inhibiting Duox expression. The airway peroxidases, lactoperoxidase (LPO) and myeloperoxidase (MPO), can detoxify pyocyanin using Duox-derived hydrogen peroxide. (Adapted from refs. and 47)
See this image and copyright information in PMC

References

    1. Leto TL. The respiratory burst oxidase. In: Gallin JI, Snyderman R, editors. Inflammation. Basic principles and clinical correlates. Lippincott Williams and Wilkins; Philedelphia: 1999. pp. 769–786.
    1. Segal BH, Leto TL, Gallin JI, Malech HL, Holland SM. Genetic, biochemical, and clinical features of chronic granulomatous disease. Medicine (Baltimore) 2000;79:170–200. - PubMed
    1. Finkel T. Reactive oxygen species and signal transduction. IUBMB Life. 2001;52:3–6. - PubMed
    1. Geiszt M, Leto TL. The Nox family of NAD(P)H oxidases: host defense and beyond. J Biol Chem. 2004;279:51715–51718. - PubMed
    1. Lambeth JD. NOX enzymes and the biology of reactive oxygen. Nat Rev Immunol. 2004;4:181–189. - PubMed

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