A single-tube, real-time PCR assay for detection of the two newly characterized human KI and WU polyomaviruses
- PMID: 18980861
- DOI: 10.1016/j.jcv.2008.09.006
A single-tube, real-time PCR assay for detection of the two newly characterized human KI and WU polyomaviruses
Abstract
Background: Three new human polyomaviruses have been recently described, and investigating their in vivo biology and pathogenicity will require sensitive and rational detection assays.
Objectives: To develop and evaluate a sensitive and rational assay for detection of the newly identified KI and WU polyomaviruses.
Study design: A single-tube, dual-probe, real-time PCR assay for simultaneous detection and discrimination of KI and WU polyomaviruses was developed.
Results: The assay had near single-molecule sensitivity for both viruses and no cross-reactivity was observed. A panel of 637 nasopharyngeal aspirates was screened, resulting in a frequency of 1.4% for KIPyV and 1.3% for WUPyV.
Conclusions: The dual-probe assay provides a rational approach for further studies of KIPyV and WUPyV.
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