Immunohistochemical features of a feline spinal cord gemistocytic astrocytoma

Abstract

A gemistocytic astrocytoma is described in the spinal cord of a 3-year-old, spayed, female, Domestic Shorthair cat. The neoplasm was tested for glial fibrillar acidic protein (GFAP), epidermal growth factor receptor (EGFR), and p53 protein expression. On histologic examination, the neoplastic cells were pleomorphic, with distinct cell borders and abundant cytoplasm that frequently extended into variably sized fibrillar processes. Neoplastic cells were strongly positive for GFAP and negative for EGFR. Eight percent (mean percentage) of neoplastic cells were p53 positive. Only 1 p53-positive cell was found in the normal spinal cord. The increased expression of p53 in the neoplasm was suggestive of an abnormal biological behavior of the p53 protein and its possible involvement in the neoplastic process of feline astrocytoma.