Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2008:14:2016-24.
Epub 2008 Nov 7.

Proteomic analysis of protein deposits on worn daily wear silicone hydrogel contact lenses

Zhenjun Zhao  1 , Xiaojia Wei, Yulina Aliwarga, Nicole A Carnt, Qian Garrett, Mark D P Willcox
Affiliations

Proteomic analysis of protein deposits on worn daily wear silicone hydrogel contact lenses

Zhenjun Zhao et al. Mol Vis. 2008.

Abstract

Purpose: Previous studies have demonstrated deposition of tear proteins onto worn contact lenses. In this study, we used proteomic techniques to analyze the protein deposits extracted from worn daily wear silicone hydrogel contact lenses in combination with different lens care solutions.

Methods: Worn lenses were collected and protein deposits extracted using urea and surfactant. Protein extracts were desalted, concentrated, and then separated using one-dimensional gel electrophoresis. Individual protein components in extracts were identified using liquid chromatography combined with tandem mass spectrometry (LC-MS-MS) after trypsin digestion.

Results: One-dimensional gel electrophoresis revealed that lysozyme and other small proteins (around 20 kDa) were the most abundant proteins in the extracts. LC-MS-MS revealed a wide array of proteins in lens extracts with lysozyme and lipocalin 1 being the most commonly identified in deposit extracts.

Conclusions: Worn contact lenses deposit a wide array of proteins from tear film and other sources. Protein deposit profiles varied and were specific for each contact lens material.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Examples of SDS gel analysis of the protein extracts. Lane 1 shows the balafilcon A lens with Opti-Free extract. Lane 2 shows the lotrafilcon B lens with ClearCare extract, and lane 3 shows the senofilcon A lens with ClearCare extract. Lane 4 shows galyfilcon A lens with Opti-Free extract. Band A is the tear lysozyme, and Band B shows unidentified proteins.
See this image and copyright information in PMC

References

    1. Stapleton F, Keay L, Jalbert I, Cole N. The epidemiology of contact lens related infiltrates. Optom Vis Sci. 2007;84:257–72. - PubMed
    1. Willcox MD, Holden BA. Contact lens related corneal infections. Biosci Rep. 2001;21:445–61. - PubMed
    1. Jones LW, Jones DA. Non-inflammatory corneal complications of contact lens wear. Cont Lens Anterior Eye. 2001;24:73–9. - PubMed
    1. Dumbleton K. Noninflammatory silicone hydrogel contact lens complications. Eye Contact Lens. 2003;29:S186–9. - PubMed
    1. Fonn D. Targeting contact lens induced dryness and discomfort: what properties will make lenses more comfortable. Optom Vis Sci. 2007;84:279–85. - PubMed

Publication types

MeSH terms

LinkOut - more resources