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. 1977 Jul;34(1):30-3.
doi: 10.1128/aem.34.1.30-33.1977.

Effect of storage time and temperature on the survival of Clostridium botulinum spores in acid media

T E Odlaug, I J Pflug

Effect of storage time and temperature on the survival of Clostridium botulinum spores in acid media

T E Odlaug et al. Appl Environ Microbiol. 1977 Jul.

Abstract

Clostridium-botulinum type A and type B spores were stored in tomato juice (pH 4.2) and citric acid-phosphate buffer (pH 4.2) at 4, 22, and 32 degrees C for 180 days. The spore count was determined at different intervals over the 180-day storage period. There was no significant decrease in the number of type A spores in either the tomato juice or citric acid-phosphate buffer stored for 180 days at 4, 22, and 32 degrees C. The number of type B spores did not decrease when storage was at 4 degrees C, but there was an approximately 30% decrease in the number of spores after 180 days of storage at 22 and 32 degrees C.

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References

    1. Appl Environ Microbiol. 1976 Nov;32(5):711-5 - PubMed
    1. Appl Environ Microbiol. 1976 Jul;32(1):121-4 - PubMed
    1. N Engl J Med. 1976 Sep 30;295(14):770-2 - PubMed
    1. Appl Environ Microbiol. 1977 Jul;34(1):23-9 - PubMed

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