doi: 10.1016/j.mimet.2008.10.006.
Epub 2008 Oct 27.
Multiplex PCR for the discrimination of A. fumigatus, A. flavus, A. niger and A. terreus
Affiliations
- PMID: 18992777
- DOI: 10.1016/j.mimet.2008.10.006
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Multiplex PCR for the discrimination of A. fumigatus, A. flavus, A. niger and A. terreus
J Microbiol Methods.
2009 Feb.
Abstract
Aspergillus pathogens usually infect immunocompromised patients with lethal outcome. We report a multiplex PCR assay for the discrimination of the most frequent Aspergillus pathogens, A. fumigatus, A. flavus, A. niger and A. terreus, through distinct amplicons of 250 bp, 200 bp, 150 bp and 450 bp respectively, derived from the rDNA gene of A. terreus and the aspergillopepsin genes of the remaining species.
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