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. 2009 Feb;76(2):209-11.
doi: 10.1016/j.mimet.2008.10.006. Epub 2008 Oct 27.

Multiplex PCR for the discrimination of A. fumigatus, A. flavus, A. niger and A. terreus

M Logotheti  1 , A Kotsovili-Tseleni, G Arsenis, N I Legakis
Affiliations

Multiplex PCR for the discrimination of A. fumigatus, A. flavus, A. niger and A. terreus

M Logotheti et al. J Microbiol Methods. 2009 Feb.

Abstract

Aspergillus pathogens usually infect immunocompromised patients with lethal outcome. We report a multiplex PCR assay for the discrimination of the most frequent Aspergillus pathogens, A. fumigatus, A. flavus, A. niger and A. terreus, through distinct amplicons of 250 bp, 200 bp, 150 bp and 450 bp respectively, derived from the rDNA gene of A. terreus and the aspergillopepsin genes of the remaining species.

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