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. 2003 Jul;42(2):101-7.
doi: 10.1023/B:CYTO.0000009919.62901.27.

Preparation of anti-2,4-dichlorophenol and 2,4-dichlorophenoxyacetic acid monoclonal antibodies

Hiroyuki Tanaka  1 , Shayu Yan, Norio Miura, Yukihiro Shoyama
Affiliations

Preparation of anti-2,4-dichlorophenol and 2,4-dichlorophenoxyacetic acid monoclonal antibodies

Hiroyuki Tanaka et al. Cytotechnology. 2003 Jul.

Abstract

The ratios of hapten and bovine serum albumin (BSA) in an antigen conjugate were determined by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Hybridomas secreting monoclonal antibodies against 2,4-dichlorophenoxyacetic acid (2,4-D) were produced by fusing 2,4-D-BSA conjugate-immunized splenocytes with a HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A substantial cross-reaction was observed for 2,4-dichlorophenol (2,4-DP) when compared with that observed for 2,4-D. The full measurement range for this assay is 0.2-3 mug ml(-1) for 2,4-DP. On the other hand, the range for 2,4-D is between 1 and 20 mug ml(-1).

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References

    1. Anastassiades M., Schwack W. Analysis of carbendazim, benomyl, thiophanate methyl and 2,4-dichlorophenoxyacetic acid in fruits and vegetables after supercritical fluid extraction. J. Chromatogr. A. 1998;825(1):45–54. doi: 10.1016/S0021-9673(98)00691-8. - DOI - PubMed
    1. Erlanger B.F., Borek F., Beiser S.M., Lieberman S. Steroid-protein conjugates I. Preparation and characterization of conjugates of bovine serum albumin with testosterone and with cortisone. J. Biol. Chem. 1957;228:713–727. - PubMed
    1. Fukuda N., Tanaka H., Shoyama Y. Formation of monoclonal antibody against a major ginseng component, ginsenoside Rg1 and its characterization. Cytotechnology. 2000;34(3):197–204. doi: 10.1023/A:1008162703957. - DOI - PMC - PubMed
    1. Fukuda N., Tanaka H., Shoyama Y. Applications of ELISA, Western blotting and immunoaffinity concentration for survey of ginsenosides in crude drugs of Panax species and traditional Chinese herbal medicines. Analyst. 2000;125(8):1425–1429. doi: 10.1039/b002932g. - DOI - PubMed
    1. Fukuda N., Tanaka H., Shoyama Y. Isolation of the pharmacologically active saponin ginsenoside Rb1 from ginseng by immunoaffinity column chromatography. J. Nat. Prod. 2000;63(2):283–285. doi: 10.1021/np990356s. - DOI - PubMed