Manipulation of cells through elastic films
- PMID: 19002987
- PMCID: PMC3449893
- DOI: 10.1023/A:1008127406783
Manipulation of cells through elastic films
Abstract
Manipulation of cells in open dishes is often incompatible withpreservation of sterility. A dish covered with an elastic orstretchable latex or plastic film allows manipulation of cellsthrough the film with preservation of sterility of the cultureand the integrity of the film. The latter forms a ;microglove'for the instrument tip. The idea of manipulation through a thintransparent film is also applicable to general surgery, so thatthe surgeon's hand operates through a film and without a glove.
References
-
- Bakker DCA, van Blitterswijk WT, Daems, Grote JJ. Biocompatibility of six elastomers in vitro. J Biomed Mater Res. 1988;22:433–439. - PubMed
-
- Carson J, Booth F. Effect of serum and mechanical stretch on skeletal alpha-actin gene regulation in cultured primary muscle cells. Amer J Physiol. 1998;275:1438–1448. - PubMed
-
- Freshney RI. A Manual of Basic Technique. New York: Alan Liss, Inc.; 1987. Culture of Animal Cells.
-
- Ingram A, Ly H, Thai K, Kang M, Scholey J. Activation of mesangial cell signaling cascades in response to mechanical strain. Kidney International. 1999;55:476–485. - PubMed
-
- Matsumoto T, Kawakami M, Kuribayashi K, Takenaka T, Tamaki T. Cyclic mechanical stretch stress increases the growth rate and collagen synthesis of nucleus pulposus cells in vitro. Spine. 1999;24:315–319. - PubMed
LinkOut - more resources
Full Text Sources
