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. 2007 Jun;54(2):71-6.
doi: 10.1007/s10616-007-9082-3. Epub 2007 Jun 16.

Electroporation: an arsenal of application

Ti-Fei Yuan  1
Affiliations

Electroporation: an arsenal of application

Ti-Fei Yuan. Cytotechnology. 2007 Jun.

Abstract

Electroporation is a way to induce nanometersized membrane pore for exogenous substances delivery into cytoplasm using an artificial electric field. Now it was widely used for molecules transfer especially in molecular experiments and genetic aspects. In recent years, modern electroporation on the embryo was developed, whose most important point is that it adopts low energy and rectangular pulse that could obtain high transfection efficiency and low damage to the embryo. This paper reviewed on the pool of application: from lab works to human clinical treatments.

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References

    1. {'text': '', 'ref_index': 1, 'ids': [{'type': 'PubMed', 'value': '10529429', 'is_inner': True, 'url': 'https://pubmed.ncbi.nlm.nih.gov/10529429/'}]}
    2. Araki I, Nakamura H (1999) Engrailed defines the position of dorsal di-mesencephalic boundary by repressing diencephalic fate. Development 126:5127–5135 - PubMed
    1. {'text': '', 'ref_index': 1, 'ids': [{'type': 'DOI', 'value': '10.1073/pnas.96.17.9885', 'is_inner': False, 'url': 'https://doi.org/10.1073/pnas.96.17.9885'}, {'type': 'PMC', 'value': 'PMC22305', 'is_inner': False, 'url': 'https://pmc.ncbi.nlm.nih.gov/articles/PMC22305/'}, {'type': 'PubMed', 'value': '10449789', 'is_inner': True, 'url': 'https://pubmed.ncbi.nlm.nih.gov/10449789/'}]}
    2. Akamatsu W, Okano HJ, Osumi N, Inoue T, Nakamura S, Sakakibara S, Miura M, Matsuo N, Darnell RB, Okano H (1999) Mammalian ELAV-like neuronal RNA-binding proteins Hub and Huc promote neuronal development in both the central and the peripheral nervous systems. Proc Natl Acad Sci USA 96:9885–9890 - PMC - PubMed
    1. None
    2. Arnold WM, Zimmermann U (1982) Rotating-field induced rotation and measurement of the membrane capacitance of single mesophyll cells of Avena sativa. Z Naturforsch 37c:908–915
    1. {'text': '', 'ref_index': 1, 'ids': [{'type': 'DOI', 'value': '10.1038/276620a0', 'is_inner': False, 'url': 'https://doi.org/10.1038/276620a0'}, {'type': 'PubMed', 'value': '723944', 'is_inner': True, 'url': 'https://pubmed.ncbi.nlm.nih.gov/723944/'}]}
    2. Baker PF, Knight DE (1978) Calcium-dependent exocytosis in bovine adrenal medullary cells with leaky plasma membrane. Nature 276:620–622 - PubMed
    1. {'text': '', 'ref_index': 1, 'ids': [{'type': 'DOI', 'value': '10.1016/S0022-1759(00)00242-8', 'is_inner': False, 'url': 'https://doi.org/10.1016/s0022-1759(00)00242-8'}, {'type': 'PubMed', 'value': '10986394', 'is_inner': True, 'url': 'https://pubmed.ncbi.nlm.nih.gov/10986394/'}]}
    2. Baron S, Poast J, Rizzo D, McFarland E, Kieff E (2000) Electroporation of antibodies, DNA, and other macromolecules into cells: a high efficient method. J Immunol Med 242:115–126 - PubMed