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. 2005 Sep;49(1):67-70.
doi: 10.1007/s10616-005-5414-3.

A Simple and Quick Method to Isolate Nuclear Extracts from Pupae of Drosophila melanogaster

Haruhisa Kawasaki  1 , Susumu Hirose, Hitoshi Ueda
Affiliations

A Simple and Quick Method to Isolate Nuclear Extracts from Pupae of Drosophila melanogaster

Haruhisa Kawasaki et al. Cytotechnology. 2005 Sep.

Abstract

Preparation of nuclear extracts is a critical step for biochemical identification of factors which function in the nuclei such as transcription factors. We have established a new method to prepare nuclear extracts from prepupae or pupae of Drosophila melanogaster. The method is simple and particularly useful for small-scale isolation from materials that are hard to get in large amounts such as specific tissues or animal bodies covered by pupal cases.

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