Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2006 Mar;50(1-3):141-62.
doi: 10.1007/s10616-005-5507-z. Epub 2006 Jun 30.

Cell culture processes for the production of viral vectors for gene therapy purposes

James N Warnock  1 , Otto-Wilhelm Merten, Mohamed Al-Rubeai
Affiliations

Cell culture processes for the production of viral vectors for gene therapy purposes

James N Warnock et al. Cytotechnology. 2006 Mar.

Abstract

Gene therapy is a promising technology for the treatment of several acquired and inherited diseases. However, for gene therapy to be a commercial and clinical success, scalable cell culture processes must be in place to produce the required amount of viral vectors to meet market demand. Each type of vector has its own distinct characteristics and consequently its own challenges for production. This article reviews the current technology that has been developed for the efficient, large-scale manufacture of retrovirus, lentivirus, adenovirus, adeno-associated virus and herpes simplex virus vectors.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Al-Rubeai M., Emery A.N., Chalder S., Jan D.C. Specific monoclonal antibody productivity and the cell cycle-comparisons of batchcontinuous and perfusion cultures. Cytotechnology. 1992;9:85–97. doi: 10.1007/BF02521735. - DOI - PubMed
    1. Al-Rubeai M., Rookes S., Emery A. N. Studies of cell proliferation and monoclonal antibody synthesis and secretion in alginate-entrapped hybridoma cells. In: de Bont J.A.M., Visser J., Mattiasson B., Tramper J., editors. Physiology of Immobilized cells. 10-12-1989. AmsterdamThe Netherlands: Elsevier Science Publishers Cells; 1990. pp. 181–188.
    1. Beer C., Buhr P., Hahn H., Laubner D., Wirth M. Gene expression analysis of murine cells producing amphotropic mouse leukaemia virus at a cultivation temperature of 32 and 37 °C. J. Gen. Virol. 2003a;84:1677–1686. doi: 10.1099/vir.0.18871-0. - DOI - PubMed
    1. Beer C., Meyer A., Muller K., Wirth M. The temperature stability of mouse retroviruses depends on the cholesterol levels of viral lipid shell and cellular plasma membrane. Virology. 2003b;308:137–146. doi: 10.1016/S0042-6822(02)00087-9. - DOI - PubMed
    1. Benihoud K., Yeh P., Perricaudet M. Adenovirus vectors for gene delivery. Curr. Opin. Biotechnol. 1999;10:440–447. doi: 10.1016/S0958-1669(99)00007-5. - DOI - PubMed