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. 2008 May;57(1):37-44.
doi: 10.1007/s10616-007-9118-8. Epub 2008 Feb 27.

Insect cells respiratory activity in bioreactor

Marilena Martins Pamboukian  1 , Soraia Athie Calil Jorge, Mariza Gerdulo Santos, Adriana Yurie Yokomizo, Carlos Augusto Pereira, Aldo Tonso
Affiliations

Insect cells respiratory activity in bioreactor

Marilena Martins Pamboukian et al. Cytotechnology. 2008 May.

Abstract

Specific respiration rate ( [Formula: see text]) is a key parameter to understand cell metabolism and physiological state, providing useful information for process supervision and control. In this work, we cultivated different insect cells in a very controlled environment, being able to measure [Formula: see text]. Spodoptera frugiperda (Sf9) cells have been used through virus infection as host for foreign protein expression and bioinsecticide production. Transfected Drosophila melanogaster (S2) cells can be used to produce different proteins. The objective of this work is to investigate respiratory activity and oxygen transfer during the growth of different insect cells lines as Spodoptera frugiperda (Sf9), Drosophila melanogaster (S2) wild and transfected for the expression of GPV and EGFP. All experiments were performed in a well-controlled 1-L bioreactor, with SF900II serum free medium. Spodoptera frugiperda (Sf9) cells reached 10.7 x 10(6) cells/mL and maximum specific respiration rate ([Formula: see text]) of 7.3 x 10(-17) molO(2)/cell s. Drosophila melanogaster (S2) cells achieved 51.2 x 10(6) cells/mL and [Formula: see text] of 3.1 x 10(-18) molO(2)/cell s. S2AcGPV (expressing with rabies virus glycoprotein) reached 24.9 x 10(6) cells/mL and [Formula: see text] of 1.7 x 10(-17) molO(2)/cell s, while S2MtEGFP (expressing green fluorescent protein) achieved 15.5 x 10(6) cells/mL and [Formula: see text] = 1.9 x 10(-17) molO(2)/cell s. Relating to the Sf9, S2 cells reached higher maximum cell concentrations and lower specific respiration rate, which can be explained by its smaller size. These results presented useful information for scale-up and process control of insect cells.

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Figures

Fig. 1
Fig. 1
DO, DO action controller, concentration of viable cells and viability cellular with the Sf9 cells in function of the time
Fig. 2
Fig. 2
Measures of OUR and formula image in liquid phase balance and specific growth cell rate with the Sf9 cells in function of the time
Fig. 3
Fig. 3
DO, DO action controller, concentration of viable cells and viability cellular with the S2 cells in function of the time
Fig. 4
Fig. 4
Measures of OUR and formula image in the liquid phase balance and specific growth cell rate with the S2 cells in function of the time
Fig. 5
Fig. 5
DO, DO action controller, concentration of viable cells and viability cellular with the S2AcGPV cells in function of the time
Fig. 6
Fig. 6
Measures of OUR and formula image in the liquid phase balance and specific growth cell rate with the S2AcGPV cells in function of the time
Fig. 7
Fig. 7
DO, DO action controller, concentration of viable cells and viability cellular with the S2MtEGFP cells in function of the time
Fig. 8
Fig. 8
Measures of OUR and formula image in the liquid phase balance and specific growth cell rate with the S2MtEGFP cells in function of the time
See this image and copyright information in PMC

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