Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2009 Jan;47(1):227-9.
doi: 10.1128/JCM.01518-08. Epub 2008 Nov 12.

Effect of ThinPrep preparation on human papillomavirus detection and genotyping in rectal samples by PCR

Sepehr N Tabrizi  1 , Matthew P Stevens, Marina T van Leeuwen, Claire M Vajdic, Leon P Botes, Gabriele Medley, Richard Hillman, Andrew Grulich, Suzanne M Garland
Affiliations

Effect of ThinPrep preparation on human papillomavirus detection and genotyping in rectal samples by PCR

Sepehr N Tabrizi et al. J Clin Microbiol. 2009 Jan.

Abstract

Specimen-to-specimen carryover during ThinPrep slide preparation was evaluated by comparing human papillomavirus genotypes detected prior and subsequent to the ThinPrep processing of 121 PreservCyt samples. Overall, 52 samples generated concordant genotypes and 38 had additional and 21 had fewer genotypes postprocessing. PreservCyt samples should be aliquoted for PCR testing prior to ThinPrep processing.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Representative cohort of samples showing the differences seen with the banding pattern observed pre- and post-ThinPrep processing.
FIG. 2.
FIG. 2.
Overall HPV genotyping results from pre- and post-TP2000 processed samples.
See this image and copyright information in PMC

References

    1. Atypical Squamous Cells of Undetermined Significance/Low-Grade Squamous Intraepithelial Lesions Triage Study (ALTS) Group. 2000. Human papillomavirus testing for triage of women with cytologic evidence of low-grade squamous intraepithelial lesions: baseline data from a randomized trial. J. Natl. Cancer Inst. 92397-402. - PubMed
    1. Bernstein, S. J., L. Sanchez-Ramos, and B. Ndubisi. 2001. Liquid-based cervical cytologic smear study and conventional Papanicolaou smears: a metaanalysis of prospective studies comparing cytologic diagnosis and sample adequacy. Am. J. Obstet. Gynecol. 185308-317. - PubMed
    1. Eltoum, I. A., D. C. Chhieng, D. R. Crowe, J. Robertson, G. Jin, and T. R. Broker. 2007. Significance and possible causes of false-negative results of reflex human papillomavirus infection testing. Cancer 11154-159. - PubMed
    1. Lentrichia, B. B., S. S. Hecht, D. Lapen, and M. K. Corkill. 1998. Potential for routine concurrent determination of chlamydia and cervical abnormalities by single fluid-based sampling. Prim. Care Update Ob. Gyns. 5149-150. - PubMed
    1. Solomon, D., M. Schiffman, and R. Tarone. 2001. Comparison of three management strategies for patients with atypical squamous cells of undetermined significance: baseline results from a randomized trial. J. Natl. Cancer Inst. 93293-299. - PubMed

Publication types

MeSH terms

LinkOut - more resources