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. 2008 Nov 13;359(20):2177-9.
doi: 10.1056/NEJMc0804021.

MRSA USA300 clone and VREF--a U.S.-Colombian connection?

Cesar A Arias, Sandra Rincon, Shahreen Chowdhury, Ernesto Martínez, Wilfrido Coronell, Jinnethe Reyes, Sreedhar R Nallapareddy, Barbara E Murray

MRSA USA300 clone and VREF--a U.S.-Colombian connection?

Cesar A Arias et al. N Engl J Med. .
No abstract available

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Figures

Figure 1
Figure 1
Panel A, Pulsed field gel electrophoresis (PFGE) (SmaI), origin, toxin profile, presence of arcA as a marker of the ACME (arginine catabolic mobile element) island and MLST of representative isolates of CA-MRSA from Colombia and the USA. SCCmec, staphylococcal chromosomal cassette mec; ST, sequence type; ND, not done. aUSA300-0114 strain, SCCmec IVa; bUSA300 strain carrying the SCCmec IVb; cthe presence of arcA has been associated with USA300 isolates carrying the SCCmec type IVa cassette but not others, consistent with the fact that ST-8 Colombian CA-MRSA may have acquired the SCCmec DNA independently from the US isolates; dSingle nucleotide variant (SNV) of ST8 in the yqiL gene. Panel B, PFGE electrophoresis using ApaI enzyme of representative isolates of vancomycin-resistant Enterococcus faecalis (VREfs) ST2 from seven hospitals in Bogotá, DC, Colombia from 2001 to 2006 and isolate TX2486, the index isolate of strain HV1 (ST2) recovered from a Houston hospital in 1994. Lane 1, TX2486; lane 2 and 3, ERV-25 and ERV-31 which are representatives of the first Colombian VREfs isolates recovered in 2001. VREfs isolates from 2002 and 2003 had an identical PFGE pattern to that of isolate ERV-31 and are not shown in the figure. Lane 4, 5 and 6, isolates ERV-62, ERV-63 and ERV-65 recovered in 2004; lane 7, ERV-81 isolated in 2005; lane 8, ERV-116, recovered in 2006.
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