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Comment
. 2008 Nov 18;105(46):17593-4.
doi: 10.1073/pnas.0810029105. Epub 2008 Nov 13.

Rolling out DNA nanostructures in vivo

Paul J Paukstelis  1 , Andrew D Ellington
Affiliations
Comment

Rolling out DNA nanostructures in vivo

Paul J Paukstelis et al. Proc Natl Acad Sci U S A. .
No abstract available

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig. 1.
Fig. 1.
In vivo replication and assembly of DNA nanostructures. (A) The in vivo replication scheme used by Lin et al. (7). dsDNA sequences corresponding to either an immobile Holliday junction or a paranemic cross-over molecule (green) were cloned into a phagemid vector and transformed into E. coli cells. Infection by a helper phage initiated in vivo rolling circle replication of ssDNAs encoding the nanostructures. (Left) Isolated ssDNAs are assayed for their ability to fold into the desired structures. (Right) Alternatively, site-selective cross-linking using trimethylpsoralen (TMP; red) provided the first demonstration that an immobile Holliday junction nanostructure could assemble within E. coli host cells. (B) Possible future applications of in vivo DNA nanotechnology. Using a system similar to that described by Lin et al., it may possible to generate in vivo replicating nanostructures that can assemble into higher-order structures through intermolecular base-pairing (orange). By including as part of the nanostructure an aptamer that can recognize, say, a membrane-associated protein (red), it may be possible to generate an “artifical cytoskeleton” that organizes the cell surface.
See this image and copyright information in PMC

Comment on

  • In vivo cloning of artificial DNA nanostructures.
    Lin C, Rinker S, Wang X, Liu Y, Seeman NC, Yan H. Lin C, et al. Proc Natl Acad Sci U S A. 2008 Nov 18;105(46):17626-31. doi: 10.1073/pnas.0805416105. Epub 2008 Oct 16. Proc Natl Acad Sci U S A. 2008. PMID: 18927233 Free PMC article.

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