Activities of mitochondrial enzymes during aerobic synchronous growth of aerobically and anaerobically grown Saccharomyces cerevisiae

Abstract

The mitochondrial enzymes cytochrome-c:O2-oxidoreductase (E.C.1.9.3.1), NADH:cytochrome-c-oxidoreductase (E.C.1.6.2.1), NADH: ferricyanide oxidoreductase (E.C.1.6.2.99), L-malate hydrolase (E.C.4.2.1.2) and L-malate:NADH-oxidoreductase (E.C.1.1.3.7) increase their activities during the aerobic synchronous growth of aerobically grown Saccharomyces cerevisiae in discrete steps and only once during the cell cycle. An identical phenomenon was observed during the aerobic synchronous growth of anaerobically grown yeast. The mechanism of completization of mitochondrial membranes is thus likely to be discontinuous and the same during both mitochondrial multiplication and the conversion of promitochondria to fully functioning mitochondria.