Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1991 Feb;59(2):387-96.
doi: 10.1016/S0006-3495(91)82232-9.

Two-dimensional crystals of streptavidin on biotinylated lipid layers and their interactions with biotinylated macromolecules

S A Darst  1 , M Ahlers, P H Meller, E W Kubalek, R Blankenburg, H O Ribi, H Ringsdorf, R D Kornberg
Affiliations

Two-dimensional crystals of streptavidin on biotinylated lipid layers and their interactions with biotinylated macromolecules

S A Darst et al. Biophys J. 1991 Feb.

Abstract

Streptavidin forms two-dimensional crystals when specifically bound to layers of biotinylated lipids at the air/water interface. The three-dimensional structure of streptavidin determined from the crystals by electron crystallography corresponds well with the structure determined by x-ray crystallography. Comparison of the electron and x-ray crystallographic structures reveals the occurrence of free biotin-binding sites on the surface of the two-dimensional crystals facing the aqueous solution. The free biotin-binding sites could be specifically labeled with biotinylated ferritin. The streptavidin/biotinylated lipid system may provide a general approach for the formation of two-dimensional crystals of biotinylated macromolecules.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1984 May;81(10):3249-53 - PubMed
    1. Biochemistry. 1987 Dec 1;26(24):7974-9 - PubMed
    1. Biochemistry. 1989 Oct 3;28(20):8214-21 - PubMed
    1. J Mol Biol. 1988 Sep 5;203(1):269-73 - PubMed
    1. Proc Natl Acad Sci U S A. 1989 Apr;86(7):2190-4 - PubMed

Publication types

LinkOut - more resources