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. 2009 Feb;53(2):835-8.
doi: 10.1128/AAC.01233-08. Epub 2008 Nov 17.

In vitro susceptibility of field isolates of Leishmania donovani to Miltefosine and amphotericin B: correlation with sodium antimony gluconate susceptibility and implications for treatment in areas of endemicity

Dhiraj Kumar  1 , Arpita Kulshrestha, Ruchi Singh, Poonam Salotra
Affiliations

In vitro susceptibility of field isolates of Leishmania donovani to Miltefosine and amphotericin B: correlation with sodium antimony gluconate susceptibility and implications for treatment in areas of endemicity

Dhiraj Kumar et al. Antimicrob Agents Chemother. 2009 Feb.

Abstract

Indian Leishmania donovani isolates (n = 19) from regional zones representing various levels of antimony resistance displayed significantly (P < 0.01) correlated results with respect to in vitro susceptibility to the antileishmanial drugs sodium antimony gluconate, amphotericin B, and Miltefosine, raising the possibility of cross-resistance mechanisms operating in the field isolates. The results of gene expression analysis of LdMT and LdRos3 were suggestive of alternate mechanisms of Miltefosine susceptibility in the isolates.

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Figures

FIG. 1.
FIG. 1.
Expression of LdMT and LdRos3 in different field isolates. (a) Real-time reverse transcription-PCR expression analysis of an L. donovani MIL transporter (LdMT). The graph shows the expression index, defined as ratios of gene expression relative to that of strain LdAG83. (b) Real-time reverse transcription-PCR expression analysis of an L. donovani MIL transporter (LdRos3). The graph shows the expression index, defined as ratios of gene expression relative to that of strain LdAG83. Data represent the means of the results of three independent experiments.
See this image and copyright information in PMC

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